HEAT SHOCK PROTEIN 90B; RBX-500UL

Analysis Note

ControlPOSITIVE CONTROL: MAD109 cells or breast carcinoma.

Application

Research CategoryProtein Trafficking

This Anti-Heat Shock Protein 90β Antibody is validated for use in IP, WB, IC, IH(P) for the detection of Heat Shock Protein 90β.

Immunoblotting: 5 µg/mL

Immunohistochemistry (frozen and formalin/paraffin): 5-10 µg/mL. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Immunocytochemistry

Immunoprecpitation: 10 µg/mg of protein lysate.

Optimal working dilutions must be determined by end user.

Research Sub CategoryChaperones

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Synthetic peptide corresponding to amino acids 2-13 from the N-terminus of mouse heat shock protein 90b.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified immunoglobulin. Liquid in 10 mM PBS, pH 7.4 with 0.2% BSA and 15 mM sodium azide.

Specificity

Reacts with a protein of 84 kD identified as heat shock protein 90beta (HSP90beta). No reactivity with HSP90alpha/HSP86.

CELLULAR LOCALIZATION: Nuclear and Cytoplasmic.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months.